Brevin Horvath

Major: Biomedical Engineering
Purdue School of Engineering and Technology

Cloning Recombinant DNA to Study the Biology of Plasmodium Falciparum

For my LHSI internship, I prepared biological tools to assist in the study of Plasmodium Falciparum, which is the parasite that causes malaria. My work consisted of cloning recombinant DNA that allowed for the study of specific genes in the parasite. I worked on preparing these biological tools for the study of a gene that is essential for the formation of the parasite’s nuclear envelope. In addition to cloning this recombinant DNA, I worked on a secondary project of recording and editing video lab protocols to assist with training new lab personnel. This video protocol library consisted of videos demonstrating how to clone DNA, and has already been used to train new rotational students in the lab. Throughout this internship, I developed skills in medical research and solidified my plans on attending graduate school to become a physician scientist.

Supervisor: Dr. Sabrina Absalon
Department: Pharmacology and Toxicology